Publications
Study summary: We combined single-cell RNA-sequencing atlases and computational analyses to map FAP-mediated signaling in homeostatic and regenerating skeletal muscle, then used FAP-depletion experiments to assess the biological relevance of predicted pathways.
Mesenchymal Stromal Cell-Mediated Intercellular Communication: Mapping the Interactome for Skeletal Muscle Homeostasis and Regeneration
Xingyu Liu, Edgar E. Perez Carbajal, Yih-Chii Hwang, Sahil A. Mapkar, Benjamin W. Gilman, Sarah A. Bliss, Lam B. Tran, Kalgi T. Mehta, Jacob O. Banyasz, Ming Yu, Reynold R. Liu, Matthew N. Ly, Christapher S. Morrissey, and Michael N. Wosczyna
Advanced Science. 2026;13(46). DOI: 10.1002/advs.202507444
Study summary: We developed a machine-learning approach using nuclear morphometrics to identify senescent cells at single-cell resolution and map age-associated senescence patterns in regenerating skeletal muscle and osteoarthritic articular cartilage.
Nuclear morphometrics coupled with machine learning identifies dynamic states of senescence across age
Sahil A. Mapkar, Sarah A. Bliss, Edgar E. Perez Carbajal, Sean H. Murray, Zhiru Li, Anna K. Wilson, Vikrant Piprode, You Jin Lee, Thorsten Kirsch, Katerina S. Petroff, Fengyuan Liu, and Michael N. Wosczyna
Nature Communications. 2025;16:6231. DOI: 10.1038/s41467-025-60975-z
Selected Prior Work
Targeting microRNA-mediated gene repression limits adipogenic conversion of skeletal muscle mesenchymal stromal cells
Michael N. Wosczyna, Edgar E. Perez Carbajal, Mark W. Wagner, Silvana Paredes, Colin T. Konishi, Ling Liu, Theodore T. Wang, Rachel A. Walsh, Qiang Gan, Christapher S. Morrissey, and Thomas A. Rando
Cell Stem Cell. 2021 Jul 1;28(7):1323–1334.e8
DOI: https://doi.org/10.1016/j.stem.2021.04.008
A single-cell transcriptomic atlas characterizes ageing tissues in the mouse
Tabula Muris Consortium
Nature. 2020 Jul;583(7817):590–595
DOI: https://doi.org/10.1038/s41586-020-2496-1
Mesenchymal Stromal Cells Are Required for Regeneration and Homeostatic Maintenance of Skeletal Muscle
Michael N. Wosczyna, Colin T. Konishi, Edgar E. Perez Carbajal, Theodore T. Wang, Rachel A. Walsh, Qiang Gan, Mark W. Wagner, and Thomas A. Rando
Cell Reports. 2019 May 14;27(7):2029–2035.e5
DOI: https://doi.org/10.1016/j.celrep.2019.04.074
Single-cell transcriptomics of 20 mouse organs creates a Tabula Muris
Tabula Muris Consortium
Nature. 2018 Oct;562(7727):367–372
DOI: https://doi.org/10.1038/s41586-018-0590-4
Bioengineered Viral Platform for Intramuscular Passive Vaccine Delivery to Human Skeletal Muscle
Nicole K. Paulk, Katja Pekrun, Gregory W. Charville, Katie Maguire-Nguyen, Michael N. Wosczyna, Jianpeng Xu, Yue Zhang, Leszek Lisowski, Bryan Yoo, Jose G. Vilches-Moure, Gordon K. Lee, Joseph B. Shrager, Thomas A. Rando, and Mark A. Kay
Molecular Therapy – Methods & Clinical Development. 2018 Sep 21;10:144–155
DOI: https://doi.org/10.1016/j.omtm.2018.06.001
A Muscle Stem Cell Support Group: Coordinated Cellular Responses in Muscle Regeneration
Michael N. Wosczyna and Thomas A. Rando
Developmental Cell. 2018 Jul 16;46(2):135–143
DOI: https://doi.org/10.1016/j.devcel.2018.06.018
Multipotent progenitors resident in the skeletal muscle interstitium exhibit robust BMP-dependent osteogenic activity and mediate heterotopic ossification
Michael N. Wosczyna, Arpita A. Biswas, Catherine A. Cogswell, and David J. Goldhamer
Journal of Bone and Mineral Research. 2012 May;27(5):1004–1017
DOI: https://doi.org/10.1002/jbmr.1562
Identification of progenitor cells that contribute to heterotopic skeletogenesis
Vitali Y. Lounev, Rageshree Ramachandran, Michael N. Wosczyna, Masakazu Yamamoto, Andrew D. A. Maidment, Eileen M. Shore, David L. Glaser, David J. Goldhamer, and Frederick S. Kaplan
The Journal of Bone and Joint Surgery. American Volume. 2009 Mar 1;91(3):652–663